Signal Transduction Reagents and Kits
Promega PYK2 Kinase Enzyme System, 1mg
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega ADP-Glo™ Kinase Assay + TOPK Kinase Enzyme System
Recombinant full-length human TOPK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TOPK is a MAPK kinase that phosphorylates p38 MAPK and is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro.
Promega p38 alpha Kinase Enzyme System, 10microg
Recombinant full-length human p38alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38alpha (SAPK2A) is a member of the p38 MAPK family, which are activated by various environmental stresses and proinflammatory cytokines.
Promega RSK1 Kinase Enzyme System, 10microg
Recombinant full-length human RSK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK1 is a member of the RSK (ribosomal S6 kinase) family that consists of growth factor-regulated serine/threonine kinases.
Promega PKA Kinase Enzyme System, 2,500 units
Recombinant bovine cAMP-Dependent Protein Kinase (PKA), Catalytic Subunit, is expressed and purified from E. coli. PKA does not require cAMP for activity, and the gene sequence can be found at accession number NM_174584.
Promega ADP-Glo™ Kinase Assay + TBK1 Kinase Enzyme System
Recombinant full-length human TBK1 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases.
Promega MARK1 Kinase Enzyme System, 1mg
Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction.
Promega ADP-Glo™ Kinase Assay + PYK2 Kinase Enzyme System
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega ADP-Glo™ Kinase Assay + FMS Kinase Enzyme System
Recombinant human FMS (539-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FMS is a proto-oncogene that encodes the tyrosine kinase transmembrane receptor for colony stimulating factor 1 receptor (CSF1R).
Promega AXL Kinase Enzyme System, 1mg
Recombinant human AXL (amino acids 473-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. AXL is a member of the receptor tyrosine kinase family, which has oncogenic potential and is implicated in human myeloid leukemia.
Promega ROCK2 Kinase Enzyme System, 10microg
ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element.
Promega ADP-Glo™ Kinase Assay + MELK Kinase Enzyme System
Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MELK or maternal embryonic leucine zipper kinase is a member of the Snf1/AMPK kinase family.
Promega CAMK4 Kinase Enzyme System, 1mg
Recombinant full-length human CAMK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK4 is a multifunctional serine/threonine protein kinase and a member of Ca2+/calmodulin-dependent protein kinase family.
Promega CDK5/p25 Kinase Enzyme System, 10microg
Recombinant full-length human CDK5 and p25 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.
Promega PKC alpha Kinase Enzyme System, 1mg
Recombinant full-length human PKC alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC alpha is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.